Strain specific IL1RAcP expression analysis in homozygous B-hIL1RAcP mice by flow cytometry. Macrophages and monocytes were collected from wild type and homozygous B-hIL1RAcP mice (H/H) and analyzed by flow cytometry with species-specific anti-IL1RAcP antibody. Human IL1RAcP were exclusively detectable in homozygous B-hIL1RAcP mice but not in wild type mice.

Splenocytes were collected from wild-type mice and homozygous B-hIL1RAcP mice and stimulated with IL1/IL33/IL36 protein. The expression of cytokines such as IL6, TNFa, KC/GRO, IL4, IFNg, IL5, and IL12p70 in splenocytes culture medium was detected after 48 hours. The results showed that murine IL1/IL33/IL36 protein effectively activated splenocytes in wild mice and triggered the secretion of related cytokines. Murine IL1B and murine IL33 proteins could effectively activate splenocytes in B-hIL1RAcP mice and trigger the secretion of related cytokines, while murine IL36 protein could not effectively activate B-hIL1RAcP mice splenocytes.