NOD.CB17-PrkdcscidIl2rgtm1BcgenIl3tm1(IL3)BcgenCsf2tm1(CSF2)BcgenCsf1tm1(CSF1)BcgenThpotm1(THPO)Bcgen/Bcgen • 112479
| Product name | huHSC-B-NDG MGMT3 mice |
|---|---|
| Catalog number | 112479 |
| Strain name | NOD.CB17-PrkdcscidIl2rgtm1BcgenIl3tm1(IL3)BcgenCsf2tm1(CSF2)BcgenCsf1tm1(CSF1)BcgenThpotm1(THPO)Bcgen/Bcgen |
| Strain background | B-NDG |
| NCBI gene ID | 1435,1437,3561,3562,5591,7066 (Human) |
| Aliases | CD132; CIDX; IL-2RG; IMD4; P64; SCIDX; SCIDX1; CSF; GMCSF; CSF-1; MCSF; DNA-PKC; DNA-PKcs; DNAPK; DNAPKc; DNPK1; HYRC; HYRC1; IMD26; XRCC7; p350; IL-3; MCGF; MULTI-CSF; MGDF; MKCSF; ML; MPLLG; THCYT1; TPO; GMCSF; CSF-1; MCSF; IL-3; MULTI-CSF; MGDF; TPO; CD132; SCIDX1; DNA-PKC |
| Application | Promote differentiation of human myeloid cells；Irradiation is not required to avoid damage to mice caused by irradiation |
Human CD34+ HSCs (3E4) were intravenously (temporal vein) engrafted into wild-type B-NDG mice and homozygous B-NDG MGMT3 mice (both sex, 24-72 hr after birth, n=15). B-NDG mice were treated with 1.0 Gy-irradiation. B-NDG MGMT3 mice were not irradiated. (A) survival rates of the mice were analyzed with Kaplan Meier survival curves. (B) Body weight. Results showed that the survival rate of B-NDG MGMT3 mice was similar to that of B-NDG mice until 18 weeks after human CD34+ HSCs engraftment and then decreased to 42.85% at 24 weeks post engraftment. But the body weight of B-NDG MGMT3 mice was significantly higher than that of B-NDG mice and increased steadily during the whole reconstitution. Values are expressed as mean ± SEM. HSCs: hematopoietic stem cells.
Human CD34+ HSCs (3E4) were intravenously (temporal vein) engrafted into wild-type B-NDG mice and homozygous B-NDG MGMT3 mice (both sex, 24-72 hr after birth, n=15). B-NDG mice were treated with 1.0 Gy-irradiation. B-NDG MGMT3 mice were not irradiated. Peripheral blood lymphocytes from the two mice after engraftment with human CD34+ HSCs were analyzed with flow cytometry. Results showed that the proportion of CD45+ cells in B-NDG MGMT3 mice reached 25% starting from 12 weeks after engraftment and continued to rise, significantly higher than that in B-NDG mice. The proportions of monocytes, MDSCs, DCs and Tregs in B-NDG MGMT3 mice were higher than that in B-NDG mice. Values are expressed as mean ± SEM.
Human CD34+ HSCs (3E4) were intravenously (temporal vein) engrafted into wild-type B-NDG mice and homozygous B-NDG MGMT3 mice (both sex, 24-72 hr after birth, n=15). B-NDG mice were treated with 1.0 Gy-irradiation. B-NDG MGMT3 mice were not irradiated. Peripheral blood lymphocytes from the two mice after engraftment with human CD34+ HSCs were analyzed with flow cytometry. Results showed that the cell numbers of all the cells analyzed from 12 weeks after engraftment in B-NDG MGMT3 mice were higher than that in B-NDG mice. Values are expressed as mean ± SEM.
