Description
- Tumor Necrosis Factor (TNF), a pleiotropic pro-inflammatory cytokine belonging to the TNF superfamily, is involved in the regulation of diverse biological processes. The AU-rich element (ARE) located within the 3' UTR of Tnfα destabilizes mRNA under steady-state conditions.
- In B-Tnf△ARE mice, the deletion of this ARE sequence enhances the stability of Tnfα mRNA, leading to chronically elevated levels of TNF protein.
- The ARE sequence in the 3'UTR of the mouse Tnf gene was deleted via gene editing, while all other sequences remained unchanged.
- Under SPF housing conditions, the serum TNF protein level in B-Tnf△ARE mice is relatively low, at approximately 3 pg/mL, whereas no expression is detectable in wild‑type mice.
- Under LPS-induced conditions, serum TNF protein levels were elevated in both B-Tnf△ARE mice and wild-type (WT) mice; however, the protein levels in B-Tnf△ARE mice were significantly higher than those in the WT group. Over time, the TNF expression in WT mice rapidly returned to basal levels, whereas B-Tnf△ARE mice maintained sustained high levels for a prolonged period.
- This product model is expected to serve as a valuable tool for research into ileitis and arthritis, functioning as a robust disease model.
- Homozygous mice are expected to exhibit severe phenotypes, resulting in embryonic lethality or poor postnatal survival; heterozygotes are suitable for research applications
Targeting strategy
Gene targeting strategy for B-Tnf△ARE mice. The ARE sequence in the 3'UTR of the mouse Tnf gene was deleted via gene editing, while all other sequences remained unchanged.
Protein expression analysis in serum
Mouse TNF expression analysis in C57BL/6JNifdc mice and heterozygous B-Tnf△ARE mice by ELISA. Serum was collected from C57BL/6JNifdc mice and heterozygous B-Tnf△ARE mice (Female, n=3). Expression level of mouse TNF were analyzed by ELISA (mouse TNF, Biolegend, 430904). Mouse TNF was detectable in heterozygous B-Tnf△ARE mice. Values are expressed as mean ± SEM.
Protein expression analysis in serum under LPS stimulation
Mouse TNF expression analysis in C57BL/6JNifdc mice and heterozygous B-Tnf△ARE mice by ELISA. Serum was collected from C57BL/6JNifdc mice and heterozygous B-Tnf△ARE mice (Female, n=3), 1h, 3h, and 6h after LPS stimulation (20 ug/200 ul. i.p.). Expression level of mouse TNF were analyzed by ELISA (mouse TNF, Biolegend, 430907). Mouse TNF was detectable in heterozygous B-Tnf△ARE mice.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Tnf△ARE mice] (Cat# 114474) was purchased from Biocytogen.
