• IL17A is mainly secreted by immune cells such as Th17, Tc17, NK cells. IL17A can form homodimers or heterodimers with IL17F. IL17A/F activates downstream signaling pathways by binding to receptors, inducing gene expression of pro-inflammatory factors (such as TNF, IL-6), chemokines (such as CXCL1). Participate in host defense and tissue repair in a healthy state; Overactivation can lead to autoimmune diseases and inflammation in diseases. IL17A/IL17F is closely related to various autoimmune diseases such as psoriasis, ankylosing spondylitis, and rheumatoid arthritis, and is a key therapeutic target for these diseases.
• The genome of mouse IL17a encoding the full-length protein was replaced by  the corresponding human IL17A genome in B-hIL17A mice(C).
• Human IL17A protein and RNA were detectable in homozygous B-hIL17A mice(C).
• This product is used for pharmacological and safety evaluation of autoimmune diseases such as psoriasis and arthritis.

Gene targeting strategy for B-hIL17A mice(C). The exons 1-3 of mouse Il17a gene that encodes the whole molecule (ATG to STOP codon) were replaced by human counterparts in B-hIL17A mice(C). The promoter, 5’UTR and 3’UTR region of the mouse gene were retained. The human IL17A expression was driven by endogenous mouse Il17a promoter, while mouse Il17a gene transcription and translation will be disrupted.

Species specific analysis of IL17A gene expression in wild-type BALB/c mice and homozygous humanized B-hIL17A mice(C) by RT-PCR. Colon and spleen were collected from wild-type BALB/c mice (+/+) and homozygous B-hIL17A mice(C) (H/H) stimulated with anti-mouse CD3ε antibody (7.5 μg/200uL, i.p.) and anti-mouse CD28 antibody (4 μg/200uL, i.p.) in vivo for 2 hours. Mouse Il17a mRNA was detectable only in wild-type BALB/c mice. Human IL17A mRNA was detectable only in homozygous B-hIL17A mice(C), but not in wild-type BALB/c mice. The positive band was confirmed to be correct by sequencing.

Strain specific IL17A expression analysis in wild-type BALB/c mice and homozygous humanized B-hIL17A mice(C) by ELISA. Serum was collected from wild-type BALB/c mice (+/+) and homozygous B-hIL17A mice(C) (H/H) stimulated with anti-mouse CD3ε antibody (7.5 μg/200uL, i.p.) and anti-mouse CD28 antibody (4 μg/200uL, i.p.) in vivo for 2 hours (female, 9-week-old, n=3). Expression level of mouse and human IL17A were analyzed by ELISA (anti-mouse IL17A antibody: ProteinTech, KE10020; anti-human IL17A antibody: Biolegend, 433917). Mouse IL17A was detectable in wild-type BALB/c mice. Human IL17A was detectable in homozygous B-hIL17A mice(C). Values are expressed as mean ± SEM.