• SLC2A12, encoding GLUT12, is a transporter protein, whose function is critical for glucose uptake in prostate cancer cells. Besides, GLUT12 also functions as a urate transporter and its dysfunction could lead to the elevation of urate concentration in blood.
• The exons 1-5 of mouse Slc2a12 gene that encode the whole molecule (ATG to STOP codon), including the 3’UTR region were replaced by human counterparts in B-hSLC2A12 mice. The promoter and 5’UTR region of the mouse gene are retained. The human SLC2A12 expression is driven by endogenous mouse Slc2a12 promoter, while mouse Slc2a12 gene transcription and translation will be disrupted.
• This mouse model can be used for mechanistic investigations of prostate cancer and the evaluation of therapeutics efficacy targeting hyperuricemia.

Gene targeting strategy for B-hSLC2A12 mice. The exons 1-5 of mouse Slc2a12 gene that encode the whole molecule (ATG to STOP codon), including the 3’UTR region were replaced by human counterparts in B-hSLC2A12 mice. The promoter and 5’UTR region of the mouse gene are retained. The human SLC2A12 expression is driven by endogenous mouse Slc2a12 promoter, while mouse Slc2a12 gene transcription and translation will be disrupted.

Strain specific analysis of SLC2A12 mRNA expression in wild-type C57BL/6JNifdc mice and B-hSLC2A12 mice by RT-PCR. Kidney RNA were isolated from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hSLC2A12 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SLC2A12 primers. Mouse Slc2a12 mRNA was only detectable in wild-type mice. Human SLC2A12 mRNA was exclusively detectable in homozygous B-hSLC2A12 mice but not in wild-type mice.