Gene targeting strategy for B-hFLT3LG mice. The exons 2~7 of mouse Flt3lg gene that encode the extracellular domain and the transmembrane domain were replaced by human FLT3LG exons 2~7 in B-hFLT3LG mice.

Strain specific FLT3LG expression analysis in homozygous B-hFLT3LG mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hFLT3LG mice (H/H). Mouse FLT3LG was detectable in wild-type mice (A). Human FLT3LG was exclusively detectable in homozygous B-hFLT3LG mice (B).

Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6N mice (female, 8-week-old, n=3) and homozygous B-hFLT3LG mice (female, 8-week-old, n=3). A. Flow cytometry analysis of the blood cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hFLT3LG mice were similar to those in C57BL/6N mice. Values are expressed as mean ± SEM.

Frequency of leukocyte subpopulations in blood by flow cytometry. Blood cells were isolated from wild-type C57BL/6N mice (female, 8-week-old, n=3) and homozygous B-hFLT3LG mice (female, 8-week-old, n=3). A. Flow cytometry analysis of the blood cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hFLT3LG mice were similar to those in C57BL/6N mice. Values are expressed as mean ± SEM.

Frequency of leukocyte subpopulations in bone marrow by flow cytometry. Bone marrow cells were isolated from wild-type C57BL/6N mice (female, 8-week-old, n=3) and homozygous B-hFLT3LG mice (female, 8-week-old, n=3). A. Flow cytometry analysis of the bone marrow cells was performed to assess the frequency of leukocyte subpopulations. Percentages of B cells, NK cells, neutrophils, dendritic cells, and monocytes in B-hFLT3LG mice were similar to those in C57BL/6N mice. Values are expressed as mean ± SEM.