Gene targeting strategy for B-hα4β7 mice.

The exons 2-27 of mouse Itga4 gene that encode the extracellular domain were replaced by human ITGA4 exons 2-27 in B-hITGA4 mice.

The exons 2-14 of mouse Itgb7 gene that encode the extracellular domain were replaced by human ITGB7 exons 3-15 in B-hITGB7 mice.

This mouse model was developed by breeding the B-hITGA4 mice and the B-hITGB7 mice.

Strain specific ITGA4 and ITGB7 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hα4β7 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H;H/H). Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse LPAM-1/α4β7 antibody (Biolegend, 120607), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB7 antibody (Invitrogen, MA5-23541) by flow cytometry. Mouse ITGA4 and ITGB7 were only detectable in wild-type C57BL/6 mice. Human ITGA4 was exclusively detectable in homozygous B-hα4β7 mice, but not in wild-type C57BL/6 mice.

Strain specific ITGA4 and ITGB7 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hα4β7 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H;H/H). Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse LPAM-1/α4β7 antibody (Biolegend, 120607), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB7 antibody (Invitrogen, MA5-23541) by flow cytometry. Mouse ITGA4 and ITGB7 were only detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice, but not in wild-type C57BL/6 mice.

Strain specific ITGA4 and ITGB7 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hα4β7 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H;H/H). Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse LPAM-1/α4β7 antibody (Biolegend, 120607), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB7 antibody (Invitrogen, MA5-23541) by flow cytometry. Mouse ITGA4 and ITGB7 were only detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice, but not in wild-type C57BL/6 mice.

Strain specific ITGA4 and ITGB7 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hα4β7 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H;H/H). Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse LPAM-1/α4β7 antibody (Biolegend, 120607), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB7 antibody (Invitrogen, MA5-23541) by flow cytometry. Mouse ITGA4 and ITGB7 were only detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice, but not in wild-type C57BL/6 mice.

Strain specific ITGA4 and ITGB7 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hα4β7 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H;H/H). Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse LPAM-1/α4β7 antibody (Biolegend, 120607), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB7 antibody (Invitrogen, MA5-23541) by flow cytometry. Mouse ITGA4 and ITGB7 were only detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice, but not in wild-type C57BL/6 mice.

Strain specific ITGA4 and ITGB7 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hα4β7 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H;H/H). Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse LPAM-1/α4β7 antibody (Biolegend, 120607), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB7 antibody (Invitrogen, MA5-23541) by flow cytometry. Mouse ITGA4 and ITGB7 were only detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice, but not in wild-type C57BL/6 mice.

Strain specific ITGA4 and ITGB7 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hα4β7 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hα4β7 mice (H/H;H/H). Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse LPAM-1/α4β7 antibody (Biolegend, 120607), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB7 antibody (Invitrogen, MA5-23541) by flow cytometry. Mouse ITGA4 and ITGB7 were only detectable in wild-type C57BL/6 mice. Human ITGA4 and ITGB7 were exclusively detectable in homozygous B-hα4β7 mice, but not in wild-type C57BL/6 mice.