B-hPD-1 plus/hPD-L1/hPD-L2 mice

C57BL/6-Pdcd1tm3(PDCD1)Bcgen Cd274tm1(CD274)Bcgen Pdcd1lg2tm3(PDCD1LG2)Bcgen/Bcgen • 112710

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B-hPD-1 plus/hPD-L1/hPD-L2 mice

Catalog Number: 112710
Strain Name: C57BL/6-Pdcd1tm3(PDCD1)Bcgen Cd274tm1(CD274)Bcgen Pdcd1lg2tm3(PDCD1LG2)Bcgen/Bcgen
Strain Background: C57BL/6
NCBI gene ID: 5133,29126,80380 (Human)
Aliases: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; ADMIO4; AIMTBS; B7-H; B7H1; PDL1; PD-L1; ADMIO5; hPD-L1; PDCD1L1; PDCD1LG1; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
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B-hPD-1 plus/hPD-L1/hPD-L2 mice

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  • Phenotypic analysis

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      mRNA expression analysis in humanized B-hPD-1 plus/hPD-L1/hPD-L2 mice

      Species specific analysis of PD-L2 gene expression in wild-type C57BL/6 mice and homozygous humanized B-hPD-1 plus/hPD-L1/hPD-L2 mice by RT-PCR. Thymus was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1 plus/hPD-L1/hPD-L2 mice (H/H). Mouse PD-L2 mRNA was detectable only in wild-type C57BL/6 mice. Human PD-L2 mRNA was only detectable in homozygous B-hPD-1 plus/hPD-L1/hPD-L2 mice, but not in wild-type C57BL/6 mice.

      Protein expression analysis in spleen

      Strain specific PD-L2 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hPD-1 plus/hPD-L1/hPD-L2 mice by flow cytometry. Bone marrow was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1 plus/hPD-L1/hPD-L2 mice (H/H) and cultured in vitro to induce the bone marrow-derived dendritic cells (BMDCs). The successfully induced BMDCs were stimulated with or without LPS in vitro. Expression of PD-L2 on BMDCs was analyzed by flow cytometry. Mouse PD-L2 was only detectable in wild-type C57BL/6 mice. Human PD-L2 was exclusively detectable in homozygous B-hPD-1 plus/hPD-L1/hPD-L2 mice, but not in wild-type C57BL/6 mice.

      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hPD-1 plus/hPD-L1/hPD-L2 mice] (Cat# 112710) was purchased from Biocytogen.