Description
- Background: Complement component C3 is a 190 kDa protein that is the most prevalent complement protein in serum and is involved in the production of fragments C3a and C3b, which are crucial for complement activation and immune response. It is also necessary for all three complement-activating pathways: classical, lectin, and alternative.
- Gene targeting strategy: The exons 2-40 of mouse C3 gene were knocked out in B-C3 KO mice. As a result, mouse C3 protein not expressed anymore.
- Validation: Mouse C3 was only detectable in wild-type C57BL/6JNifdc mice but not in B-C3 KO mice.
Targeting strategy
Gene targeting strategy: The exons 2-40 of mouse C3 gene were knocked out in B-C3 KO mice. As a result, mouse C3 protein not expressed anymore.
Protein Expression Analysis in Aerum
Strain specific mouse C3 expression analysis in wild-type C57BL/6JNifdc mice and B-C3 KO mice by ELISA. Serum was collected from wild-type C57BL/6JNifdc mice (+/+) (male, n=3, 8-week-old) and B-C3 KO mice (-/-) (male, n=3, 8-week-old). The expression level of mouse C3 were analyzed by ELISA (mouse C3 ELISA kit: Abcam, ab157711). Mouse C3 was only detectable in wild-type C57BL/6JNifdc mice but not in B-C3 KO mice.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-C3 KO mice] (Cat# 114102) was purchased from Biocytogen.
