Description
- Function research of genes
- This Drd2Cre model is an efficient tool to study various gene functions when crossed with rats with different loxP site-flanked genes of interest, especially in studies of D2 dopamine receptor subtype-expressing neurons, and may be useful for studying dopamine-sensitive behaviors such as addiction.
Gene editing strategy
A P2A-NLS-Cre sequence cassette was placed between the coding sequence
of exon 3 and 3’UTR of the Drd2 gene.
This strain was maintained on a Sprague Dawley genetic background.
Phenotype Analysis
k Immunofluorescent images of tdTomato and DRD2 in the striatum of Drd2 reporter rats. Arrows indicate the colocalization of tdTomato and DRD2. Scale bar, 25 µm. l Western blot of DRD2 and GAPDH in the striatum from Drd2::Cre+/− and WT rats. Left, representative blots, right, quantifcation results. NS not signifcant, n= 3, t test (source: Yu Q et al.,2019)
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Drd2-Cre rats] (Cat# 210488) was purchased from Biocytogen.
