Gene targeting strategy for B-hAPLNR mice. The exon 1 of mouse Aplnr gene that encodes the full-length protein was replaced by human APLNR exon 1 in B-hAPLNR mice.

Anti-human APLNR antibody binding assessment in B-hAPLNR MC38. B-hAPLNR MC38 and MC38 were analyzed by flow cytometry. Anti-hAPLNR antibody hAPLNR Ab-A (provided by a client) can bind B-hAPLNR MC38.

Anti-human APLNR antibody binding assessment in B-hAPLNR mice. Spleen were collected from wild-type C57BL/6N mice (+/+) (male, 6-week-old) and homozygous B-hAPLNR mice (H/H) (male, 6-week-old) and analyzed by flow cytometry. Anti-hAPLNR antibody hAPLNR Ab-A (provided by a client) can bind macrophages of B-hAPLNR mice.

Anti-human APLNR antibody binding assessment in B-hAPLNR mice. Liver were collected from wild-type C57BL/6N mice (+/+) (male, 6-week-old) and homozygous B-hAPLNR mice (H/H) (male, 6-week-old) and analyzed by flow cytometry. Anti-hAPLNR antibody hAPLNR Ab-A (provided by a client) can not bind macrophages of B-hAPLNR mice.

Anti-human APLNR antibody binding assessment in B-hAPLNR mice. Small intestine were collected from wild-type C57BL/6N mice (+/+) (male, 6-week-old) and homozygous B-hAPLNR mice (H/H) (male, 6-week-old) and analyzed by flow cytometry. Anti-hAPLNR antibody hAPLNR Ab-A (provided by a client) can not bind macrophages of B-hAPLNR mice.

Anti-human APLNR antibody binding assessment in B-hAPLNR mice. Brain were collected from wild-type C57BL/6N mice (+/+) (male, 6-week-old) and homozygous B-hAPLNR mice (H/H) (male, 6-week-old) and analyzed by flow cytometry. Anti-hAPLNR antibody hAPLNR Ab-A (provided by a client) can not bind macrophages of B-hAPLNR mice.

Immunohistochemical analysis of organs in B-hAPLNR mice . The main organs of B-hAPLNR mice(H/H) were isolated from female(6-week-old, n=1) and male(9-week-old,n=1). Human APLNR Antibody(LS-A64) used for IHC analysis. Considering that APLNR is a membrane protein, typical positive signals are observed in the mucosal epithelium of lung and stomach tissues. Staining in other tissues may represent nonspecific binding signals of the antibody. Scale bar: 200 μm.