CD19

• A chimeric CDS that encodes human CD19 signal peptide and extracellular domain, mouse CD19 transmembrane and cytoplasmic domain, followed by mouse 3’UTR-STOP was inserted right after mouse Cd19 exon 2. 
• The chimeric CD19 protein expression will be driven by endogenous mouse Cd19 promoter, while mouse Cd19 gene transcription and translation will be disrupted.

Strain-specific CD19 expression analysis in B-hCD19 mice by flow cytometry. Spleens were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hCD19 mice (H/+), and homozygous B-hCD19 mice (H/H), and analyzed by flow cytometry using an anti-CD19 antibody. Mouse CD19 was detectable in wild-type and heterozygous B-hCD19 mice (H/+). Human CD19 was exclusively detectable in heterozygous B-hCD19 (H/+) and homozygous B-hCD19 (H/H) mice.

Analysis of leukocyte subpopulations by flow cytometry in immune organs and blood.  Splenocytes, peripheral blood, and bone marrow were isolated from C57BL/6 mice and homozygous B-hCD19 mice (female, 8-week-old, n = 3). Single live cells were gated on the CD45⁺ population and analyzed by flow cytometry as indicated. Values are expressed as mean ± SEM.

Analysis of T-cell subpopulations by flow cytometry in immune organs and blood. Splenocytes, peripheral blood, and bone marrow were isolated from C57BL/6 mice and homozygous B-hCD19 mice (female, 8-week-old, n = 3). Single live cells were gated on the CD3⁺ T-cell population and analyzed by flow cytometry as indicated. Values are expressed as mean ± SEM.