B-hCD3EDG/hBCMA mice

C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)BcgenCd3gtm1(CD3G)Bcgen Tnfrsf17tm1(TNFRSF17)Bcgen/Bcgen • 112602

B-hCD3EDG/hBCMA mice

Product nameB-hCD3EDG/hBCMA mice
Catalog number112602
Strain nameC57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)BcgenCd3gtm1(CD3G)Bcgen Tnfrsf17tm1(TNFRSF17)Bcgen/Bcgen
Strain backgroundC57BL/6
NCBI gene ID916,915,917,608 (Human)
AliasesIMD18, T3E, TCRE; CD3-DELTA, IMD19, T3D; CD3-GAMMA, IMD17, T3G; BCM, BCMA, CD269, TNFRSF13A

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  • Phenotypic analysis

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      mRNA expression analysis

      Strain specific analysis of CD3EDG and BCMA gene expression in wild type (WT) mice and B-hCD3EDG/hBCMA mice by RT-PCR.

      Mouse Cd3edg and Bcma mRNA were detectable only in splenocytes of WT mice (+/+). Human CD3EDG and BCMA mRNA were detectable only in homozygous B-hCD3EDG/hBCMA mice (H/H; H/H) but not in WT mice (+/+). The positive band was confirmed to be correct by sequencing.

      Protein expression analysis

      Strain specific CD3E expression analysis in homozygous B-hCD3EDG/hBCMA mice by flow cytometry.

      Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3EDG/hBCMA mice (H/H; H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hBCMA mice (H/H; H/H) but not in WT mice (+/+).