Strain specific analysis of ITGB1 gene expression in wild-type mice and B-hITGB7 mice by RT-PCR. Mouse Itgb1 mRNA was detectable in splenocytes of wild-type mice (+/+). Human ITGB1 mRNA was detectable only in homozygous B-hITGB7 but not in wild-type mice.

Strain specific ITGB1 expression analysis in homozygous B-hITGB1 mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hITGB1 mice (H/H) analyzed by flow cytometry with species-specific anti-ITGB1 antibody. Mouse ITGB1 was detectable in WT mice. Human ITGB1 were exclusively detectable in homozygous B-hITGB1 mice (H/H).