Gene targeting strategy for B-hITGB1 mice. The exons 2-15 of mouse Itgb1 gene were replaced by human ITGB1 exons 2-15 in B-hITGB1 mice.

Strain specific analysis of ITGB1 gene expression in wild-type mice and B-hITGB1 mice by RT-PCR. Mouse Itgb1 mRNA was detectable in splenocytes of wild-type mice (+/+). Human ITGB1 mRNA was detectable only in homozygous B-hITGB1 but not in wild-type mice.

Strain specific ITGB1 expression analysis in homozygous B-hITGB1 mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hITGB1 mice (H/H) analyzed by flow cytometry with species-specific anti-ITGB1 antibody. Mouse ITGB1 was detectable in WT mice. Human ITGB1 were exclusively detectable in homozygous B-hITGB1 mice (H/H).