huPBMC-B-NDG MHC I/II DKO mice ad

NOD.CB17-Prkdcscid Il2rgtm1Bcgen H2-K1tm1Bcgen H2-Ab1tm1Bcgen H2-D1tm1Bcgen/Bcgen • 114316

huPBMC-B-NDG MHC I/II DKO mice ad

Product namehuPBMC-B-NDG MHC I/II DKO mice ad
Catalog number114316
Strain nameNOD.CB17-Prkdcscid Il2rgtm1Bcgen H2-K1tm1Bcgen H2-Ab1tm1Bcgen H2-D1tm1Bcgen/Bcgen
Strain backgroundB-NDG
NCBI gene ID14972,14964,14961,16186,19090 (Mouse)
AliasesK-f; H-2K; H2-K; H2-D1; H-2K(d); H-2D, H2-D, H2-K1; AI845868, Abeta, H-2Ab, H2-Ab, I-Abeta, IAb, Ia-2, Ia2, Rmcs1; gc; p64; [g]c; gamm; CD132; gamma(; gamma(c); DNA; DOX; dxn; DNA-; XRCC; p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs;AI326420; AU019811; DNA-PKcs

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  • Description
  • Targeting strategy

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      Description
      • Human PBMC-engrafted humanized mice are attractive models for in vivo analysis of human immune responses. We previously reported the successful engraftment of human PBMCs in B-NDG mice. However, due to the severe xenograft versus host disease (xeno-GvHD) in these mice, the experimental window is limited. The pathogenesis of GvHD is directly related to human T cell implantation, and the mismatch between human and mouse MHCs is the main cause of GvHD after human PBMCs engraftment. It has been shown that knocking out MHC I and/or II molecules in mice can reduce GvHD and extend the experimental window after engraftment of human PBMCs.
      • MHC class I molecules are composed of two subunits, α and β chains, and the α chain in NOD background strain of mice is encoded by H2-K1, H2-D1 gene. Knocking out H2-K1 and H2-D1 gene in mice makes it unable to form a complete MHC Class I molecule. MHC class II molecules are also composed of two subunits, α and β chains, and the β chain is encoded by H2-Ab1 gene. Knocking out H2-Ab1 gene in mice makes it unable to form a complete MHC Class II molecule. These incomplete MHC Class I and MHC Class II molecules will not be able to effectively express on the cell surface and perform antigen-presenting functions, thereby potentially reducing the occurrence of GvHD.
      • Application: This model can be used to study the mechanism of xeno-GvHD in vivo, the pathogenesis of immune-related diseases such as tumor, autoimmune and metabolic diseases, and to evaluate the efficacy of novel drugs .
      Targeting Strategy

      Gene targeting strategy for B-NDG MHC I/II DKO mice ad. The exons 2-8 of mouse H2-K1 and H2-D1 gene, the exons 2-4 of mouse H2-Ab1 gene were knocked out in B-NDG MHC I/II DKO mice ad. So the transcription and translation of mouse H2-K1H2-D1H2-Ab1 genes will be disrupted.