Phenotype Analysis
Scheme for generating Foxc2CRE/+;R26T/Gf/f mice. The Rosa26mTmGfloxmice (stock no. 007676) were crossed withFoxc2iCreERt2mice (Foxc2iCreERt2mice was purchased from Biocytogen Co. Ltd., Beijing, China) to obtain Foxc2CRE/+;R26T/Gf/fmice. Foxc2+cells were specifically labeled with GFP after tamoxifen treatment in Foxc2CRE/+;R26T/Gf/fmice.(source: Wang Z et al., 2023)
Results of agarose gel electrophoresis
Gel image of PCR products. PCR products are separated by gel electrophoresis on a 2% agarose gel. PC: samples used for PCR were collected from heterozygous mice; WT: samples used for PCR were collected from wild type mice; H2O: blank control.