C57BL/6-Ctla4tm1(CTLA4)Bcgen Tnfr2tm1(TNFR2)Bcgen/Bcgen • 111843
| Product name | B-hCTLA4/hTNFR2 mice |
|---|---|
| Catalog number | 111843 |
| Strain name | C57BL/6-Ctla4tm1(CTLA4)Bcgen Tnfr2tm1(TNFR2)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 1493,7133 (Human) |
| Aliases | ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12; CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR |
Targeting strategyGene targeting strategy for B-hCTLA4/hTNFR2 mice. The exon 2 of mouse Ctla4 gene that encodes the extracellular domain was replaced by human CTLA4 exon 2 in B-hCTLA4/hTNFR2 mice. The exons 2~6 of mouse Tnfr2 gene that encode the extracellular domain was replaced by human TNFR2 exons 2~6 in B-hCTLA4/hTNFR2 mice.
Protein expression analysis
Strain specific hCTLA4 expression analysis in homozygous B-hCTLA4/hTNFR2 mice by flow cytometry. Spleen was collected from wild-type mice (+/+) and homozygous B-hCTLA4/hTNFR2 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD152 (CTLA4) antibodies. Mouse CTLA4 was detectable in wild-type mice. Human CTLA4 was exclusively detectable in homozygous B-hCTLA4/hTNFR2 mice but not in wild-type mice.
Strain specific hTNFR2 expression analysis in homozygous B-hCTLA4/hTNFR2 mice by flow cytometry. Spleen was collected from wild-type mice (+/+) and homozygous B-hCTLA4/hTNFR2 mice (H/H), and analyzed by flow cytometry with species-specific anti-TNFR2 antibodies. Mouse TNFR2 was detectable in wild-type mice. Human TNFR2 was exclusively detectable in homozygous B-hCTLA4/hTNFR2 mice but not in wild-type mice.
