C57BL/6-Sirpatm1(SIRPA)Bcgen Cd47tm1(CD47)Bcgen Cd3etm2(CD3E)Bcgen Cd28tm1(CD28)Bcgen/Bcgen • 111874
| Product name | B-hSIRPA/hCD47/hCD3E/hCD28 mice |
|---|---|
| Catalog number | 111874 |
| Strain name | C57BL/6-Sirpatm1(SIRPA)Bcgen Cd47tm1(CD47)Bcgen Cd3etm2(CD3E)Bcgen Cd28tm1(CD28)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 140885,961,916,940 (Human) |
| Aliases | BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS1, SIRP; IAP, MER6, OA3; IMD18, T3E, TCRE; Tp44 |
Targeting strategyGene targeting strategy for B-hSIRPA/hCD47/hCD3E/hCD28 mice. The exons 2-6 of mouse Cd3e gene that encode the extracellular domain were replaced by human CD3E exons 2-7 in B-hSIRPA/hCD47/hCD3E/hCD28 mice. The exons 2-3 of mouse Cd28 gene that encode the extracellular domain were replaced by human CD28 exons 2-3 in B-hSIRPA/hCD47/hCD3E/hCD28 mice. The exon 2 of mouse Cd47 gene that encodes the IgV domain was replaced by exon 2 of human CD47 gene in B-hSIRPA/hCD47/hCD3E/hCD28 mice. The exon 2 of mouse Sirpα gene that encodes the IgV domain was replaced by exon 2 of human SIRPα in B-hSIRPA/hCD47/hCD3E/hCD28 mice.
Protein expression analysis
Strain specific CD3E expression analysis in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibodies. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H) but not in WT mice (+/+).
Strain specific CD28 expression analysis in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD28 antibodies. Mouse CD28 was detectable in WT mice (+/+). Human CD28 was exclusively detectable in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H) but not in WT mice (+/+).
Strain specific SIRPA expression analysis in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-SIRPA antibodies. Mouse SIRPA was detectable in WT mice and B-hSIRPA/hCD47/hCD3E/hCD28 mice due to the cross-reactivity of antibodies. Human SIRPA was exclusively detectable in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H) but not in WT mice (+/+).
Strain specific CD47 expression analysis in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD47 antibodies. Mouse CD47 was detectable in WT mice (+/+). Human CD47 was exclusively detectable in homozygous B-hSIRPA/hCD47/hCD3E/hCD28 mice (H/H;H/H;H/H;H/H) but not in WT mice (+/+).
